Fig. 2. DMR methylation of the Igf2r and H19 genes in offspring derived from transplantation of 8.5 and 12.5 dpc PGCs. DNA methylation was analyzed by bisulfite genomic sequencing. Both male and female offspring of each stage of donor PGCs were analyzed. Individual lines represent sequenced clones. Black ovals indicate methylated cytosine-guanine sites (CpGs) and white ovals indicate unmethylated CpGs.