Fig. 5. Kul cleaves Delta but not Serrate in the wing disc. (A) Dl expression (red) in a wild-type wing disc. (B) Overexpression of Kul in the wing pouch eliminated the normal Dl protein in the wing margin and future veins. Note that in the notum, where MS1096-GAL4 is not expressed, normal levels of Dl were retained (arrow). (C,D) Overexpression of Kul by sal-GAL4 eliminated Dl in the sal domain. (E-G) Expression of Dl (red) in flipout clones (marked by GFP expression). (H-J) Co-expression of Kul (blue) and Dl in flipout clones eliminated the Dl protein. Thus, Kul mediates directly cleavage of Dl, rather than affecting the expression of Dl. (K-M) Clones of cells expressing Kul (green) showed elimination of Dl but not Ser. The loss of Dl was confined to the clone, demonstrating the cell-autonomous activity of Kul. (N-P) Overexpression of Kuz eliminated both Dl and Ser within the clones.