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Fig. 6. retn expression during metamorphosis. retn-Gal4/+; UAS-mGFP expression in late larval (A,D,G,J), early pupal (B,E,H,K), and late pupal/early adult (C,F,I,L) stages. (A-L) Anterior is upwards. (A-C) retn expression labels subsets of CNS neurons through metamorphosis: mushroom bodies (arrowheads), subesophageal ganglion (arrows) and ventral abdominal ganglion (asterisks). (D-F) retn labels {alpha} and ß mushroom body processes in the larval CNS (D). These projections are pruned in 24-hour-old pupae (arrow in E). In 48-hour-old pupae, expression can be seen in all MB lobes, but expression subsequently fades in non-{alpha}/ß projecting neurons (F). (G-I) Subesophageal ganglion cells remain constant in number, but show remodeling of projections from larval (G) to early (H) and late (I) pupal patterns. (J-L) Eighteen larval retn-expressing abdominal ganglion cells (arrow, J) reduce to 12 in early pupae (arrow, K). Six neurons are present (arrow, L) in late pupal stages.