Fig. 5. Treatment of mIMCD-3 cells with Bmp2 increases ß-catenin/P-Smad1 molecular complexes and Myc expression. Immunoblots and quantitation are shown on the left and right panels, respectively. Treatment with recombinant 10 nM Bmp2 increased intracellular levels of ß-catenin 1.9-fold (A), ß-catenin/P-Smad1 molecular complexes 2.3-fold (B) and Myc 2.4-fold (C). (D) Bmp2 treatment of mIMCD-3 cells transfected with a plasmid encoding the Myc promoter (-1490 to -1) upstream of luciferase increased luciferase activity 10-fold by 1 hour and 26-fold by 2 hours after addition of Bmp2.