Fig. 4. Distribution of ß-catenin in the developing cartilage. Immunohistochemistry with an antibody against ß-catenin on sections of tibia from E14.5 wild-type (A,A') versus Ihh^-/- embryos (B,B'), or from E16.5 wild type (C,C') versus Ihh^-/- embryos (D,D'). (E) In situ hybridization using a ³⁵S-labeled riboprobe against Col10a1 on a section immediately adjacent to that shown in D. Boxed regions in A-D are shown at a higher magnification in A'-D'. Red arrows in A' and C' indicates nuclear staining of ß-catenin. Yellow arrow in C' marks a nucleus negative for ß-catenin staining. Arrows in C and D indicate peripheral chondrocytes with nuclear staining. Arrowheads in A', B' and D' indicate staining at the cell junctions. Asterisk in B denotes the cartilage proper. Asterisks in D mark chondrocytes with ß-catenin staining. P, proliferating chondrocytes; PH, prehypertrophic chondrocytes; EH, early hypertrophic chondrocytes; LH, late hypertrophic chondrocytes; C, columnar chondrocytes.