Fig. 7. Wnt signaling induces osteogenesis in C3H10T1/2 cells. (A) Induction of AP by Wnt3a-conditioned medium versus the control L medium. (B-F) Expression of Runx2 (B), Osx (C), Col1a1(I) (D), OP (E) and OC (F) in cells treated with either Wnt3a-conditioned medium (red bars) or the control L medium (grey bars), as determined by real-time PCR. (G-I) Partial inhibition of Smo^*-induced osteogenesis by either Dkk1 or dnTCF4. Cells were infected with Smo^* virus mixed at equal titer with either Dkk1 virus, dnTcf4 virus or a control GFP virus. AP expression was determined by both an activity assay (G) and real-time PCR (H). Bsp expression was determined by real-time PCR (I). NV, no virus.