Fig. 6. Expression of AML1-ETO and Lz-enR inhibits SME-lacZ expression in larval and pupal eye discs. The eye discs are stained for Cut (green) and ß-gal (red). Larval eye discs are shown in A,B,E,F,I,J,M,N and pupal eye discs are shown in C,D,G,H,K,L,O,P. (A-D) In wild type, SME-lacZ is expressed in cone cells, which also express cut, during larval and pupal stages (the green and red channels are separated). (E-H) SME-lacZ is directly activated by Lz, and is therefore not expressed in lz^null eye discs, which also do not have cone cells. (I-L) SME-lacZ levels are significantly reduced in lz-Gal4 UAS-AML1-ETO eye discs. This effect is more obvious at pupal stages (K,L). Cut expression remains. (M-P) Ectopic expression of Lz-enR also inhibits SME-lacZ expression (lz-Gal4 UAS-lz-enR). Cut expression remains.