Fig. 4. Cyclin E and Dacapo expression pattern in the microchaete cell lineage. Staining of nota from A101 pupae between 16 and 24 hours APF. (A-I) CycE expression in bristle lineage cells at progressive stages of development. CycE staining is in red and ß-gal staining in green. Combined time-lapse imaging of living pupae followed by CycE detection was performed in parallel to order the panel sequence. (J-R) Dap expression in bristle lineage cells at progressive stages of development. Dap staining is in red and ß-gal staining in green. In R, the neuron is identified by Elav staining (blue). (S,T) Coexpression of CycE (red) and dap (blue) at a two (S) and three-cell (T) cluster stage as shown in E and L, in N and an intermediate stage between F and G, respectively. Sensory organ cells are stained by anti-Sens immunoreactivity (green). In S and T, the overlay is on the right. (U) Temporal localisation of each panel (A-T) during the cell lineage. Scale bar: 2 µm. g, glial cell, s, sheath cell; n, neuron; sh, shaft cell; so, socket cell.