Fig. 4. Ganglion formation in Gbx2^-/- inner ears. Heterozygous (A,C,E,G) and homozygous (B,D,F,H) Gbx2 inner ears probed for Nf68 (A,B) Gata3 (C,D) and Neurod1 (E,F) transcripts, and apoptotic cells (G,H) at (A-D) 15.5, (E,F) 10.5 and (G,H) 9.5 dpc. (G,H) TUNEL analyses show an increased in apoptotic cells in the cochleo-vestibular ganglion (G,H, red arrowheads) of a –/– otocyst (H) compared with a +/– otocyst (G, black arrowheads). Arrows indicate a region of cell death within the otic epithelium of +/– and –/– inner ears. cvg, cochleo-vestibular ganglion; gg, geniculate ganglion; sg, spiral ganglion; vg, vestibular ganglion. Schematics on the right indicate the levels of sections. Scale bars: in D, 200 µm for A-D; in F, 100 µm for E,F; in H, 100 µm for G,H.