Fig. 3. path regulates growth in Drosophila. (A) Map of the path (CG3424) genomic region. Two predicted transcription units for path have been defined: RA and RC. Coding (red) and non-coding (blue) regions are indicated. roo (grey) is a naturally occurring transposable element found in path. KG06640, GS13857 and GS11111 insertions are shown in green. (B) path^KG06640 homozygotes and path^KG06640/Df(3L)AC1 transheterozygotes were reduced in size compared with wild-type controls. (C,D) Histograms show adult weights and wing sizes for different path mutant genotypes. Values for mutant flies were strongly reduced compared with wild type (P<0.001), and were significantly rescued (P<0.001; light grey bars) by both UAS-path and CG1139^GS10666 expression under arm-GAL4 control and by the presence of combined heterozygous mutations in Tsc1 and Tsc2, which produce only a very modest size increase in otherwise normal flies. (E-H) Eyes from control (E), a path^KG06640 homozygote (F), a path^KG06640/Df(3L)AC1 transheterozygote (G) and a path^KG06640 homozygote expressing UAS-path constitutively (H). (I) path mutant animals (here path^KG06640/Df(3L)AC1 transheterozygotes) were reduced in size throughout larval and pupal development, and developmentally delayed. However, path^KG06640 homozygous climbing third instar larvae had wing imaginal discs of roughly normal size (K) compared with wild type (J). Genotype of Tsc1/Tsc2-rescued flies in C and D is path^KG06640 Tsc2¹⁹²/path^KG06640 Tsc1²⁹.