Fig. 2. Alterations of the size of the neural plate in Xsix3 gain- and loss-of-function experiments. (A-H,K-Z) Embryos injected unilaterally with Xsix3 (A-H,K-N), GR-Xsix3 (O-R), VP16-Xsix3 (S-V) or MoXsix3 (W-Z), and analysed at early neurula stage. The probes used are indicated in each panel. Red staining in A,B,D-H,K-Z and turquoise staining in C represent expression of the co-injected lacZ lineage tracer. The injected side of the embryos (to the right of dotted lines representing the midline) is indicated (inj). (A-G,K-M,O-Z) Frontal views, dorsal towards the top; (H,N) ventral views (VV), anterior towards the bottom. (H,N) Arrows indicate Xk81 repression caused by Xsix3 overexpression. (I,J) Embryos untreated (I) or treated (J) with HUA, brown nuclear staining representing anti-phosphorylated histone H3 staining; dorsal views, anterior towards the bottom. (K-N) Embryos injected with Xsix3 and treated with HUA. (A,B,K) White brackets indicate the anterior expression of Sox2 (A,K) or Xnrp1 (B) in Xsix3-injected (right) and control (left) sides.