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Fig. 1. Expression of fetal Leydig cell markers in embryonic testes of single and double Sf1 and Dax1 mutants. In situ hybridization for steroidogenic enzymes Cyp17 (A-D) and Cyp11a1 (E-H) identifies the Leydig cells of the embryonic testis at 13.5 dpc. Leydig cells are present in columns between testis cords in wild-type mice (A,E). Leydig cells are decreased in Sf1+/– gonads in agreement with dosage-sensitive regulation (B,F), and are restricted to the coelomic surface of the Dax1-/Y testis (C,G). Sf1/Dax1 double mutant mice are devoid of Leydig cells markers (D,H).