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Fig. 4. Disrupted cardiac pre-pattern and Tbx2 expression in Tbx20lacZ/lacZ embryos. (A-M) Whole-mount in-situ hybridization analysis of wild-type (wt), Tbx20lacZ/lacZ, Nkx2-5GFP/GFP or compound Tbx20lacZ/lacZ/Nkx2-5GFP/GFP embryos using indicated probes. Panel C shows sections of whole-mount embryos at the level of the sinuatrium. sa, sinuatrium.