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Fig. 8. Abnormal motoneuron differentiation in Tbx20 knockdown embryos. (A) Expression of Tbx20 and motoneuron markers Isl2, Hb9 and Isl1 in sections of E9.5 embryos at the thoracic level. Isl2 and Hb9 expression is decreased in Tbx20 knockdown embryos (siTbx20) compared with wild-type embryos (wt). (B) Normal dorsoventral patterning of the spinal cord in Tbx20 knockdown embryos, as shown by expression of Irx3 and Pax6. (C) Expression of Tbx20, Isl1, Isl2 and Hb9 in overlapping domains in the spinal cord at E9.