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Fig. 4. Apoptosis and proliferation assays in Tbx20-null mutants and control littermates. (A-D) TUNEL analysis revealed no increase in apoptosis in Tbx20-null embryos relative to control littermates. Arrows indicate cardiac crescent (A,B) and heart tube (C,D). (E-L) Antibody staining (whole mount and sections) for phosphorylated histone H3 reveals decreased proliferation in Tbx20-null mutants relative to control littermates. Arrows indicate positive phosphorylated histone H3 staining in cardiomyocytes.