Fig. 8. Schematic diagram of hemocyte maturation in the lymph gland. (A) The earliest lymph gland cells, the hemocyte precursors (HP), express Srp (S⁺) and Odd (O⁺). As these cells transition into pre-prohemocyte (PPH) fate, they initiate the expression of Hemese (H⁺) and Pvr (Pv⁺). Prohemocytes (PH) initiate dome-gal4 (d_g4⁺) expression. Maturation to the various hemocyte (H) fates requires downregulation of dome-gal4 (d_g4^-), upregulation of different maturation markers (M⁺) and the involvement of the indicated signaling pathways. (B) Spatial and temporal sequence of hemocyte development in the lymph gland (see text for details). The PSC cells are marked by Collier (C⁺) and Serrate (Ser⁺) expression. Ubiquitous cell markers such as Srp, Odd, Pvr and Hemese are expressed in all the lymph gland cells marked by the nuclear marker To-pro-3, thus they are expressed in all the cell types and the zones. Mature hemocytes markers such as Pxn, P1 and Lz were compared with dome-gal4 expression. Lamellocyte marker msn-lacZ was compared with G147. All the markers used in the diagram except Collier were directly compared with Ser-lacZ. PM, plasmatocyte; CC, crystal cell; LM, lamellocyte; CZ, cortical zone; MZ, medullary zone; PSC, posterior signaling center.