Fig. 2. Phenotypes of Src42A^26-1 mutants. (A1) Size and location of the Src42A^26-1 deletion are shown by the filled box. Triangle indicates P insertion; red boxes indicate exons; H, HindIII; S, SalI. (A2,A3)Anti-Src42A antibody staining of wild-type (A2) and Src42A^26-1 (A3) embryos, indicating Src42A^26-1 a protein-null allele. (B1-B3) Src42A^26-1 embryos stained for Fas3 (B1,B2) or Engrailed (B3). These embryos were associated with slightly irregular midline (B1,B2; bottom). Staining for Engrailed (magenta) and E-cad (white) indicated cell-cell matching failure along the midline in these embryos (B3). Bottom panel provides an interpretation (magenta, Engrailed-expressing cells). (B4-F4) Functional redundancy of Src42A and Src64 found in dorsal closure/germ-band retraction (B4,B5), CNS (C1-C4), trachea (D1-D4), visual system (E1-E3) and ovary (F1-F4). Anterior is towards the left. (B4) Dorsal view (top) and enlargement of the dorsal midline region (bottom) in Src42A^26-1;Src64^P1/+ embryos. (B5) Lateral view of Src42A^26-1;Src64^P1 embryos. No germband retraction was observed. (C) CNS stained for Fas2 (top) or Elav (bottom). In Src double mutants, longitudinal axons were misrouted but neurons appeared not significantly reduced in number. (D) Embryos stained for Trachealess (Trh, top) and lumen (2A12, bottom). (E) Head regions stained for Fas2. Bolwig's organ (bo) fails to separate from the optic lobe (ol). (F) Ovaries stained with Rhodamine-phalloidin (magenta) and SYTOX (green).