Fig. 4. Src-dependent temporal change in Fas3/tubulin (A1-A9) and E-cad/Arm (B1-B9) accumulation in DME-cells during dorsal closure. Embryos were collected at times indicated. Arrows indicate leading edge. (A) In wild-type, polarized expression of Fas3 and tubulin bundling and cell-elongation along the dorsoventral axis were observed at all times. (B) Leading edge was always smooth in appearance and leading edge signals of E-cad (green) and Arm (magenta) increased gradually 9-11 hours AEL. Src42A^26-1;Src64^P1/+ embryos exhibited almost normal cell-shape change and signal deposition at 9 hours AEL (B2), but, at later stages, leading edge frequently kinked (arrowheads in B4) and simultaneously Arm and E-cad signals reduced significantly (B4,B6,B8). In B6 and B8, cytoplasmic Arm signals were found. Src42A^26-1 embryos were almost normal in cell-shape change and marker-protein deposition at the leading edge (A9,B9).