Fig. 6. Inhibition of neural crest migration by dominant-negative Wnt11 is rescued by activated Dsh. (A) Two-cell stage embryos were injected with dnWnt11 mRNA and RDX (red), or with Dsh-N and FDX (green). At stage 14, neural crest cells were taken from the Dsh-N-injected embryo and grafted into the dnWnt11-injected embryo. Analysis of neural crest migration was performed by examining Slug expression and fluorescence. (B) Control side of embryo injected with dnWnt11. Arrowheads indicate normal neural crest migration. (C) Injected side of the embryo shown in B. Asterisk indicates the absence of neural crest migration. (Inset) RDX fluorescence. (D,E) Graft of Dsh-N/FDX-expressing cells into an embryo injected with dnWnt11/RDX. (Inset) RDX fluorescence. Note the normal migration of the FDX-expressing cells. Seventy-five percent of embryos showed rescued neural crest migration; n=20.