Fig. 5. tlr is expressed in the pupal wings and is required for the Bmp signaling in the posterior crossvein. (A) Expression of tld and tlr in 30 hours AP pupal wings is compared by in situ hybridization with antisense tld and tlr DIG-labeled probes for the same length of time. Inserts show control hybridization of embryos at the early cellularization stages using the same probes. (B) Accumulation of pMad during the pupal wing development in wild-type and tlr mutant animals 19 hours after pupariation (AP), 25 hours AP and 30 hours AP. The bottom panel shows the corresponding adult wings, with the tlr mutant wing missing the posterior crossvein.