Fig. 2. Anxa5-lacZ reporter is specifically expressed in the vasculature of E10.5 embryos. Sagittal sections (A-D,F-I) of heterozygous embryos were stained for ß-galactosidase activity (A,F) followed by immunodetection of SMA (B,G), PECAM (C,H) and nuclear staining by DAPI (D,I). In the overlays (E,J), X-gal deposits are indicated by white spots. Anxa5-lacZ⁺ cells are restricted to SMA⁺/PECAM⁺ dorsal aorta and PECAM⁺ capillaries (E). At higher magnification, only a subset of PECAM-associated cells was positive for lacZ, as shown by DAPI staining (J, arrows). (K-N) Anxa5-lacZ⁺ cells are located in regions positive for the pericyte-specific marker NG2-proteoglycan in embryonic brain at E13.5. Transverse sections of capillaries were stained for ß-galactosidase activity (K, arrows). Positive areas appear black because of the monochrome character of the camera system. In parallel, PECAM (L) and NG2-proteoglycan (M) were detected by immunostaining. After merging, colocalization of PECAM and NG2 appears orange (N). X-gal deposits are shown as white spots (N, arrows). Scale bar: 50 µm.