Fig. 5. Expression profiles of isolated Anxa5-lacZ⁺ cells. Cells were sorted from heterozygous embryos at E10.5 (A), dissected brains from E16.5 embryos (B), adult brain meninges (C) and RNA was isolated. RNA isolated from total embryos at E10.5 represented the positive control (D). RT-PCR was used to detect the mRNA of specific markers for stem cells (Flk1, Kit, Sca1, CD45, CD34, VE-cadherin), myogenic cells (Myod1) as well as myogenic satellite cells (Pax7) and pericytes (PDGFRß, NG2). Anxa5 was tested as a positive control and reactions without mRNA (E) were used as negative controls. GAPDH was used for standardization.