Fig. 8. Purified Anxa5-lacZ⁺ PVCs do not have hematopoietic capacity in vivo. Purified Anxa5-lacZ⁺ PVCs from adult brain meninges (Ly5.2⁺, 1 x10⁵ cells) were mixed with bone marrow cells (Ly5.1⁺, 9 x10⁵) and injected intravenously into recipient mice (Ly5.1⁺) after lethal irradiation. After 28 days, peripheral blood of recipients was analyzed by flow cytometry for the presence of Ly5-markers. (A) No Ly5.2⁺ cells were detectable in blood of the negative control mice, represented by non irradiated wild-type Ly5.1⁺ mice without transplantation. (B) In the positive control, 18% of the blood cells derived from the injected Ly5.2⁺ BMCs. (C) No Ly5.2⁺ cells could be detected after administration of Anxa5-lacZ⁺/Ly5.2⁺ donor PVCs.