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Fig. 4. Secondary heart field development in Tbx20–/– embryos. Whole-mount in situ hybridization analysis of Tbx20 and Mlc2a expression in wild-type embryonic hearts at E7.75 (A,C) and E8.0 (B,D); of markers for OFT (Wnt11) and OFT/RV (Hand2) in E9.5 hearts (E-F'), and of secondary heart field markers in E8.5 wild-type and mutant embryos. Images are magnifications of anterior body regions, including the heart, in views from the right lateral side (E-G',I,I',K-M'), or from the ventral side (A-D,H,H',J,J'). Anterior is always up. Markers and genotypes are as indicated in the figure, for details on expression, see main text.