Fig. 6. hh contains functional SO-binding sites. (A) Genomic map of the hh locus. The physical mapping is indicated as: X, XhoI; S, SacI; K, KpnI. The hh¹ (bar-3) deletion is mapped according to Lee et al. (Lee et al., 1992). Two SO-binding sites are located within the region deleted in hh¹ (sequences are given in Fig. 4C: hh first, hh second). One additional SO-binding site is found upstream of the hh¹ deletion. (B) hh¹-lacZ: lacZ expression posterior to the MF is mediated by 1.4 kb genomic DNA from the region deleted in hh¹. (C) hh¹ SOmut: expression is hardly detectable when the two SO-binding sites are mutated. (hh first, GAG is changed to CCC; hh second, GAT is changed to CCC). (D) hh¹ 5'-lacZ: no expression is detected from a reporter construct containing only one mutated SO-binding site