Fig. 3. Comparison of reporter activity with expression of chicken SP. Stage 29 PV were transfected with pTP-1Venus reporter construct and processed for fluorescent in situ hybridization of chicken SP after 3 hours of culture. The reporter activity is observed in a scattered fashion. Venus expression and chicken SP expression do not overlap. Lower three panels are high-magnification views of the boxed areas in the upper and middle panels. Scale bar: 100 µm.