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Fig. 2. The morphology of spe-38 mutant sperm is indistinguishable from that of wild-type sperm. (A-C) White arrows indicate examples of spermatozoa in Nomarski DIC images. In vitro-activated sperm from wild-type (A) and spe-38(eb44) (B) male worms appear identical. (C) In vivo-activated hermaphrodite sperm within the spermatheca of an unmated spe-38(eb44) hermaphrodite are indistinguishable from wild-type sperm (A). Some of these sperm are in direct contact with the oocyte. (D-F) Transmission electron micrographs of spe-38(eb44) sperm within spe-38(eb44) hermaphrodites. The ultrastructural details of spe-38 sperm are indistinguishable from those of wild type, including the sperm chromatin mass (N), the pseudopod (P) and the fused membranous organelles (MOs). (F) Close-up view of MOs in spe-38(eb44) sperm highlights the fusion pore (black arrow) surrounded by an electron-dense collar. Scale bars: in A, 5 µm for A-C; 1 µm in D,E; 0.5 µm in F.