Fig. 6. Molecular cloning of the spe-38 gene. (A) Genetic and physical maps of the spe-38 region of chromosome I. Expanded views below the genetic map show the relative position of informative single nucleotide polymorphisms (SNPs), and the structure/orientation of the Y52B11A.1 (spe-38) and Y52B11A.2 genes. Exons are indicated by the blue and green boxes. The transgenic rescue profile of DNA fragments containing these genes is shown on the right. (B) Expanded view showing the intron and exon structure of the spe-38 gene. Exons are indicated by the green boxes and are numbered from right to left to keep orientation consistent with the genetic map. The 270 bp deleted in the spe-38(eb44) mutant strain are indicated by the bracket under the structural representation of the gene. (C) PCR characterization of the spe-38(eb44) mutation. Primers designed to amplify an 1.5 kb DNA fragment from wild-type worms amplify a smaller DNA fragment from spe-38(eb44) homozygous animals. Marker sizes are indicated to the right of the gel.