Fig. 6. ANI-2 localizes to the lining of the rachis of the syncytial gonad. Wild-type C. elegans syncytial gonads were fixed and stained for DNA, NMY-2 and ANI-2. Images were collected every 0.4 µm through the volume of the gonad. (A) Images of the bottom half of the gonad were projected to visualize the inside surface of the rachis and the openings to the developing oocytes (arrows in A,D). Right: schematic view of the central section of a C. elegans gonad. (B) Schematic of a cross-sectional view of the rachis comparing the localization of NMY-2 and ANI-2. Inset box shows region and orientation of view in C. (C) Higher magnification view of the pachytene region of the gonad. Nuclei are regularly spaced. The ANI-2 staining on the surface of the rachis is visible along the edges in cross-section (arrows). (D) Higher magnification view of the bend in the gonad where oocytes increase dramatically in size. The images in C and D are taken from a different gonad from the one shown in A. Scale bars: 10 µm.