Fig. 3. Synergistic activation of 3EE-GFP by eya, so and dpp. (A-C) Each set of three panels shows the same wing disc stained with anti-Dac (magenta) and anti-GFP (green) or a merge of the two channels. (A) Wing imaginal discs in which the expression of UAS-ey is driven by 30A-Gal4 show ectopic expression of 3EE-GFP and Dac in two regions at the anteroposterior (AP) compartment boundary. (B) Wing imaginal discs expressing a combination of eya and so driven by 30A-Gal4 can strongly induce 3EE-GFP and Dac at the AP compartment boundary where their expression coincides with endogenous dpp (white). (C) A combination of dpp, eya and so driven by 30A-Gal4 synergistically induces the expression of 3EE-GFP and Dac in the entire ring around the wing pouch.