Fig. 4. Increased apoptotic cell death in frizzled 9 mutants. (A) TUNEL staining at E12.5, E14.5, E18.5 and P8 mice. The dotted lines outline the forming dentate gyrus at E18.5 and the dentate granule cell layer at P8. At E12.5, note the numerous labeled cells at the exact midline where the choroid forms but very few cells in the cortical hem (CH) or ventricular zone dorsal to it. (B) Counting of individual labeled cells and clusters of cells expressed at % of wild type. ^*P<0.05; ^**P<0.005.