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Fig. 1. Targeting strategy and confirmation of genotype. (A) The genomic organization of the Pkb{gamma} wild-type allele (top) was disrupted using a targeting vector with an IRES-lacZ-Neo-cassette (middle). Targeting of the wild-type allele leads to disruption of exon 4 of the Pkb{gamma} gene (bottom). Arrowheads indicate the localization of the primers for the PCR reaction. (B) The genotype of mice was determined by PCR. Representative results from Pkb{gamma}+/+, Pkb{gamma}+/- and Pkb{gamma}-/- mice are shown. (C) The levels of PKB{gamma} in the brains of Pkb{gamma}+/+, Pkb{gamma}+/- and Pkb{gamma}-/- mice were determined by western blot analysis using a PKB{gamma}-specific antibody. (D) The levels of PKB{alpha} and PKBß, respectively, were determined in brain lysates from Pkb{gamma}+/+, Pkb{gamma}+/- and Pkb{gamma}-/- mice using PKB{alpha}- and PKBß-specific antibodies.