Fig. 3. Phospho-western blot analysis of brains from Pkb mutant mice. (A) Brains of three wild-type and three mutant mice were analysed for phosphorylation status of proteins involved in PKB signalling. p, indicates phosphorylated protein. (B) Western blot quantification. Levels of p-Ser473 and p27 were normalized to the level of actin, all phosphorylated proteins were normalized to the level of unphosphorylated protein. ^*P<0.05. Error bars represent s.d.