Fig. 7. Increased susceptibility to glutamate and staurosporine induced cell death. (A-D) Primary hippocampal neurons were established from E16.5 embryos and kept in culture for 28 days. Immunocytochemistry was performed using antibodies against dendritic (Map2C) or axonal (tau) proteins. (E) PKB, PKBß and PKB expression in Pkb wild-type and mutant hippocampal neurons. (F) Seven-day-old cultures were treated with glutamate (15 mM/24 hours) or with staurosporine (50 nM/12 hours) or were left untreated (Pkb^+/+ white bars and Pkb^-/- black bars). Apoptotic cells were identified by TUNEL-assay and at least 200 cells per culture were counted (n=5 cultures per treatment and genotype). ^*P<0.05. Error bars represent s.d.