Fig. 2. Confocal and whole-mount immunohistochemical analyses of marker gene expression and gonad morphology in B6 E12.5 and E13.5 gonad-mesonephros complexes. In each case, both marker gene expression and gonad morphology of Dax1-/Y ovaries are indistinguishable from normal Dax1+/+ ovaries. The top four rows illustrate the expression of GATA4, SF1, WT1 and SOX9 in E12.5 gonads (red staining). The bottom row illustrates AMH expression in E13.5 gonads (red staining). PECAM expression on the surface of germ cells and vascular endothelial cells is shown in each image (green staining). The left, middle and right columns are B6 Dax1+/+ ovaries, B6 Dax1-/Y ovaries and B6 Dax1+/Y testes, respectively. In each image the gonad is at the top and the mesonephros at the bottom.