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Fig. 3. Relative Sry expression in B6 Dax1-/Y and Dax1+/Y gonads as determined by real time RT-PCR analysis. y-axis indicates 18s RNA normalized Ct values. Black line illustrates the normalized threshold detection limit of the real time RT-PCR assay (Ct=37.5 - mean Ct 18s RNA). Values below this line indicate the gene is not expressed. An asterisk indicates a significant difference between B6 Dax1+/Y and B6 Dax1-/Y fetal gonads at P≤0.05.