Fig. 7. IFT88 mutations affect Gli3 processing. (A) Immunoblots show the levels of Gli3-190 and Gli3-83 in wild type, Ift88^null and Ift88^hypo mouse embryos at E10.5. In wild-type embryos, the majority of Gli3 protein is proteolytically processed to form Gli3-83, whereas in both Ift88^null and Ift88^hypo mutants, the Gli3 processing is impaired. The bands marked with asterisks in lanes 1 and 4 are nonspecific and not consistently detectable. (B) Graphical representation of the ratio of Gli3-190 versus Gli3-83 in wild type, Ift88^null and Ift88^hypo mutant embryos. The results were obtained from three independent experiments. Numbers above the bars show an average of the ratio.