Fig. 2. NKX6.1 and NKX6.2 are expressed in distinct domains of the developing pancreas. Confocal images showing immunofluorescence detection of NKX6.2 together with NKX6.1. (A,B) At E10.5, NKX6.1 and NKX6.2 together mark most of the pancreatic epithelial cells. A subset of cells in both the ventral (vp, A) and dorsal (dp, B) pancreatic anlage co-express NKX6.1 and NKX6.2 (yellow cells in A,B). At E12.5, only a few scattered cells co-express both factors (arrowheads in C). At E15.5, the domains of NKX6.1 and NKX6.2 are largely distinct (D). s, stomach. Scale bar: 20 µm.