Fig. 7. In situ hybridization for Ngn3 (A-D) and Myt1 (E-H) in pancreas from wild-type, Nkx6.2^-/-, Nkx6.1^-/- and Nkx6.1^-/-;Nkx6.2^-/- embryos at E14.5. Ngn3 expression is similar in wild type and all Nkx6 compound mutants (A-D). By contrast, expression of Myt1 is reduced in Nkx6.1^-/- and almost absent in Nkx6.1^-/-;Nkx6.2^-/- embryos (E-H). (I) Quantitative RT-PCR analysis for Myt1 mRNA in pancreata from Nkx6 compound mutants at E13.5. Myt1 levels are shown relative to the levels for HPRT mRNA from three independent measurements of three pancreata for each genotype. Values are shown as a % of wild type (arbitrarily set to 100%)±s.e.m. ^*P<0.01, as determined by Student's t-test. Scale bar: 50 µm.