Fig. 1. (A) ClustalW alignment of the predicted amino acid sequences of zebrafish
Lmx1b.1, Lmx1b.2 and chick Lmx1b. Identical residues are shaded and dashes
indicate predicted gaps. Arrows indicate the positions of the residues against
which degenerate primers were designed. (B) Matrix of amino acid sequence
comparisons among Lmx sequences from zebrafish (z), chick (c), human (hu),
mouse (m) and hamster (ha). Percentages of identical and, in brackets, similar
residues are given. (C) Phylogenetic relationships among several Lmx protein
sequences. Both zebrafish Lmx genes obtained in our screen cluster with other
Lmx1b orthologs. A putative zebrafish Lmx1a ortholog (put zLmx1a) found in the
Ensembl Zebrafish Genome Server (ENSDARP00000006532) clusters with Lmx1a
orthologs. Numbers represent bootstrap values for each branchpoint. Accession
numbers: zLmx1b.1, AY894989