Fig. 3. Cloning of the sma-5 gene, mRNA structure and RNAi experiments. (A) Physical map of the region around sma-5 gene. The YAC DNA clone, cosmid DNA clones and PCR fragments used for identification of the sma-5 gene are shown. Thick lines represent regions carrying functional sma-5 gene. (B) Location of deletions in W06B3.2 and structure of mRNAs. White and grey rectangles indicate coding and non-coding exons, respectively. (C) RNAi experiments. Average volumes of progeny born after injection of dsRNA of W06B3.2 into N2 (RNAi-1, 2, 3), control progeny born from a parent injected with water or sma-5(n678) mutant worms are shown together with standard deviations. Two-day-old adult animals were used for measurement.