Fig. 7. Effect of expression of extrachromosomal sma-5 genomic gene (second group of columns) or sma-5 cDNA under the indicated promoter (lower groups of columns) in the sma-5 (n678) mutant on body and organ volume in 2-day-old adults. Body volumes relative to those of the wild-type body volume (white bar), and intestinal volumes (gray bar) or hypodermal volumes (black bar) relative to those of the dss-1::gfp or col-19p::gfp transgenic lines in the wild-type background are shown by bars. The top group of columns show the results of the sma-5 (n678) mutant. T-shaped bars represent s.e.m., which was calculated by the formula (fDx²/y²+x²fDy²/y⁴)^1/2/n^1/2 where x and fDx are mean and s.d. of the volume of the indicated strain, and y and fDy are those of the wild-type volume (Bevington and Robinson, 2003). The smaller of the numbers of worms examined for the indicated and the wild-type worms was taken as n. An asterisk indicates significant difference in t-tests (P0.05) from the value for the sma-5 mutant. One-hundred percent (wild-type volume) corresponds to 4.39±0.41 nl, 0.88±0.068 nl and 1.01±0.20 nl for body, intestinal and hypodermal volumes, respectively. Hypodermal volume of a worm expressing sma-5 cDNA under dss-1 promoter was not obtained because a corresponding transgenic line was not obtained.