Fig. 5. Sox9 and Pax7 expression is reduced in cyclopamine-treated blastemas. (A-C) Sox9 in situ hybridization on cross-sections of control (A), cyclopamine-treated (B) and agonist-treated (C) regenerates 6 dpa. Note the absence of staining in B. The arrows in C point to Sox9-positive dorsal blastema cells. (D) Quantification of Pax7-positive blastema cells in control and cyclopamine-treated regenerates 6 dpa. Columns represent the mean percentage of Pax7-positive blastema cells of three regenerates (total number of counted cells per regenerate is between 704 and 1128). Error bars indicate standard deviations. The t-test value is 0.0007. Scale bar: 100 µm in A-C.