Fig. 2. Effects of Gli3 in neural plate patterning. Whole-mount in situ hybridizations on stage 14 embryos injected (inj.) with MO-C or MO-3 with probes as indicated on the left side of each panel. The ß-gal tracer is red except in Pintallavis-MO-3 and the BrdU panels, in which it is blue. Arrows indicate the inhibition of Gli1, Xash3, XMyt1, Xaml, Slug and Snail expressions. Double-headed arrows indicate the size of the neural plate assessed by Sox3 expression. BrdU incorporation in stage 14 embryos injected with MO-3 is shown in brown. A high magnification panel (bottom right) shows BrdU incorporation in the neural plate of a unlilaterally injected MO-3+lacZ embryo.