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Fig. 1. Dox-controlled VEGFR2T expression in hepatocytes. (A) Schematic diagram of the transgene TRE-VEGFR2T. (B) Western blotting of newborn liver using an anti-HA antibody. Lanes 1-3, TRE-VEGFR2T sub-line 4377.5; lanes 4-6, sub-line 3647.5. (C,D) Expression of tTA by lacZ reporter assay in (C) E10.5 and (D) E11.5 embryos. (E,F) Immunostaining for the extracellular domain of VEGFR2 on LAP-tTA control liver (E) and LAP-tTA/TRE-VEGFR2T mutant liver (F). Nuclei are stained blue with DAPI. (G) VEGF levels in liver by ELISA. Error bars represent s.d. *P<0.01.