Fig. 3. Phenotypic analysis of right ventricular outflow tract (rvot) defects in adult Carp-Lbh transgenic mice. (A,B) In situ lateral view of the great arteries of 6-month-old wild-type (wt) and transgenic (Tg) mice, and (C,D) transverse histological sections through the rvot of 3-month-old wt and Tg hearts stained with Hematoxylin and Eosin show pulmonary atresia (arrow) in the Tg hearts (B,D). (E,F) Cross sections of the hearts shown in A and B and close-ups on right ventricular (rv) cardiomyocytes (insets) depict biventricular hypertrophy (asterisks) in the Tg (F). (G,H) Polymer casts of 5 months-old wt and Tg mice. Mixing of pulmonary (blue) and systemic (red) blood in the ascending aorta (arrowhead) and pulmonary stenosis (arrow) are evident in the Tg (H). (I,J) Diagrams of a wt and a Tg heart with Tetralogy of Fallot (TOF) showing the association of pulmonic stenosis (PS), an overriding aorta, a patent ductus arteriosus (PDA), a ventricular septum defect (VSD) and rv hypertrophy. ao, aorta; la, left atrium; lv, left ventricle; pa, pulmonary artery; ra, right atrium.