Fig. 1. Destruction of Xlsirts or VegT, but not Xpat RNA, releases Vg1 mRNA from the cortex. (A) Stage VI oocytes analyzed by whole-mount in situ hybridization with digoxigenin-labeled Vg1 probe. (1) Control oocytes injected with antisense Xpat ODN. P, pigmented animal pole; arrows indicate non-pigmented vegetal pole with localized Vg1 mRNA. (2) Oocytes injected with antisense VegT ODN or (3) antisense Xlsirts ODNs. (B) Sections showing the fragments of vegetal cortex of control non-injected (1), antisense VegT- (2) and antisense Xlsirts- (3) injected stage VI oocytes. Arrows indicate Vg1 mRNA at the vegetal cortex in control oocyte and remnants of Vg1 mRNA at the vegetal cortex in antisense Xlsirts- and antisense VegT-injected oocytes. Scale bar: 380 µm in A; 30 µm in B. (C) Northern blot of poly(A)RNA isolated from 100 control and antisense Xpat ODN-injected oocytes, hybridized to antisense Xpat and antisense Xcat2 digoxigenin-labeled probes.