Fig. 3. Anti-pancytokeratin C11 antibody disrupts cytokeratin network in stage VI oocytes. Anti-pancytokeratin C11 antibody conjugated with FITC was injected into the cytoplasm of stage VI oocytes. At various time points, oocytes were fixed for 5 minutes in 1% formalin in PBS and vegetal cortexes were observed under fluorescent microscope. (A) Up to 30 minutes from the injection of antibody. (B) Three hours after the injection of antibody. (C) Antibody-injected progesterone matured oocyte. (D) Antibody-injected, matured and activated (by pricking) oocyte. (E) Antibody injected oocyte post-injected with rescuing VegT RNA shows partial reconstitution of the cytokeratin network. Scale bars: 12 µm.