Fig. 1. FBF and FOG-1 control larval germline proliferation. (A-C,F) DAPI-stained
germlines. Broken lines indicate germlines; small arrowheads indicate sperm;
large arrowheads indicate oocytes or oocyte-like cells; arrow in A-E indicates
distal end; vul, vulva. fog-1, fog-1(q250). (A) fog-1
homozygote. Size of adult germline is normal, but only oocytes are made
(Barton and Kimble, 1990). (B)
fbf-1 fbf-2 adult. Germline is reduced to 
60 germ cells/arm with
only sperm (Crittenden et al.,
2002). (C) fog-1; fbf-1 fbf-2 triple mutant. Germline is
reduced to 10 germ cells (white arrowheads) and no sperm are made. (D)
fog-1; fbf-1 fbf-2 triple mutant, stained with antibodies to the germ
cell marker PGL-1; few germ cells are present (white arrowheads). (E)
fog-1; fbf-1 fbf-2 Nomarski micrograph; few germ cells are present
(white arrowheads). (F) L2 fog-1; fbf-1 fbf-2. Arrow indicates
crescent-shaped nucleus typical of early meiosis. Scale bars: 10 µm. (G)
Larval germline proliferation. eL1, early L1; lL1, late L1. n
4 at
all stages.