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Fig. 4. FBF acts upstream of fog-1 in the sperm/oocyte decision. (A-D) Broken lines indicate germlines. Green indicates sperm-specific SP56 marker; red indicates oocyte-specific RME-2 marker. Genotypes, stages and markers are indicated in micrographs. (A,C) fbf-1 fbf-2 mutants express sperm marker, but not oocyte marker. (B,D) fog-1; fbf-1 fbf-2 mutants do not express sperm, but express oocyte marker. Scale bars: 10 µm. (E) Model for FBF repression of multiple genes in germline sex determination pathway.